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Categories Functional consequence Method of detection References Transcription factors Nuclear receptors activation Co-IP, in vitro 150, 151 AhR/ARNT activation Co-IP, in vitro 152– 154 AP-1 activation Yeast 2-hybrid, in vitro 155 Brn-3 activation Co-IP 156 β-catenin activation Mammalian 2-hybrid, in vitro 157 c-Ets activation Co-IP 158 E2F1 activation Co-IP, in vitro 135 HNF4 activation Yeast 2-hybrid 159, 160 IRF3 activation Yeast 2-hybrid 161 MEF-2C activation Mammalian 2-hybrid, Co-IP, in vitro 18 NF-κB activation Co-IP 162 Smad3 activation Co-IP, mammalian 1-hybrid 163 Tat activation Co-IP, in vitro 164 TEF-4 activation Yeast 2-hybrid, in vitro 17 TTF1 activation in vitro 165 Oncogene/Tumor suppressor HPV E7 suppression Co-IP, in vitro 166 p53 activation in vitro 167 Rb activation Co-IP, in vitro 168 Kinase/Phosphatase aPKC phosphorylation, stabilization Co-IP 56 c-Abl phosphoryaltion, activation Co-IP 50 GSK3 phosphorylation, activation, degradation enzyme-substrate interaction 54 IKK phosphorylation, activation Co-IP 61 MAPK phosphorylation, activation enzyme-substrate interaction 42, 69 PDXP dephosphorylation, suppression enzyme-substrate interaction 55 PKA phosphorylation, activation enzyme-substrate interaction 43, 169 PP1 dephosphorylation, stabilization enzyme-substrate interaction 55 PP2A dephosphorylation, suppression enzyme-substrate interaction 55 Ub/Sumo enzymes ARIP3/PIASxα sumoylation, activation in vitro 74 E6AP ubiquitination, degradation Co-IP, in vitro 170 Fbw7 ubiquitination, activation, degradation Co-IP 54 UBCH7 activation Co-IP, in vitro 171 Coregulators ANCOS suppression Yeast 2-hybrid, Co-IP, in vitro 172 ASC activation Yeast 2-hybrid, in vitro 173 CARM1 activation, methylation, suppression Yeast 2-hybrid, Co-IP 174 CoCoA activation Co-IP, in vitro 15 GAC63 activation Co-IP, in vitro 14 GCN5 activation Co-IP, yeast 2-hybrid 23 MMS19 activation Yeast 2-hybrid, in vitro 175 p300/CBP activation, acetylation, suppression Co-IP, in vitro 35 PGC1 activation in vitro 142 Others Cyclin T1 activation in vitro 164 FLASH activation, suppression Yeast 2-hybrid, in vitro 176, 177 GAS activation in vitro 178 JAB1 activation Yeast 2-hybrid, in vitro 179 Pin1 activation, degradation in vitro 180 REGγ degradation Co-IP 77 SRA activation IP 181 TIA, TIAR translational inhibition Co-IP, in vitro 63. 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Co-IP, coimmunoprecipitation; IP, immunoprecipitation; in vitro, in vitro protein-protein interaction assay; AhR/ARNT, aryl hydrocarbon receptor/aryl hydrocarbon receptor nuclear translocator; AP-1, activator protein 1; Brn-3, POU domain, class 4, transcription factor 1; c-Ets, mammalian protooncogene homologue of the avian v-ets; E2F1, E2F transcription factor 1; HNF4, hepatic nuclear factor 4; IRF3, interferon regulatory factor 3; MEF-2C, myelin basic protein expression factor 2; NF-κB, nuclear factor of kappa light polypeptide gene enhancer in B-cells 1; Smad3, MAD homolog 3; Tat, Human immunodeficiency virus transactivator protein; TEF-4, transcriptional enhancer factor family of transcription factors; TTF1, thyroid transcription factor 1; p53, transformation related protein 53; Rb, retinoblastoma 1; APKC, atypical protein kinase C; c-Abl, c-abl oncogene 1, receptor tyrosine kinase; GSK3, glycogen synthase kinase 3; IKK, inhibitor of kappaB kinase; MAPK, mitogen-activated protein kinase; PDXP, pyridoxal (pyridoxine, vitamin B6) phosphatase; PKA, protein kinase A; PP1, protein phosphatase 1; PP2A, protein phosphatase 2; ARIP3/PIASxα, protein inhibitor of activated STAT 2; E6AP, ubiquitin protein ligase E3A; Fbw7, F-box and WD-40 domain protein 7; UBCH7, ubiquitin-conjugating enzyme E2L 3; ANCOS, ankyrin repeats containing cofactors; ASC, Activating signal cointegrator 1; CARM1, coactivator-associated arginine methyltransferase 1; CoCoA, calcium binding and coiled coil domain 1; GAC63, solute carrier family 30 (zinc transporter), member 9; GCN5, lysine acetyltransferase 2A; MMS19, MET18 S. 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